[Stitcher]

New Fluorescent Label Sheds Light On Brain Diseases

http://www.sciencedaily.com/releases/2007/11/

ScienceDaily (Nov. 5, 2007) — In an advance that may speed progress toward new diagnostic tests for Alzheimer's disease (AD) and Parkinson's disease (AD), scientists in New York are reporting development of the first direct method for measuring a key enzyme implicated in both of those chronic brain disorders.

Dalibor Sames and Mary K. Froemming point out that the enzyme -- 17B-HSD10 -- has stirred excitement among researchers as a potential biomarker that could be used to diagnose AD and PD and chronicle the effectiveness of treatments. Other studies have found that PD patients have reduced levels of the enzyme, while increased levels seem to protect laboratory mice from the disease. 17B-HSD10 also attaches to the abnormal brain protein in AD, perhaps contributing to the loss of brain cells. "Despite the importance of this emerging physiological and pathological marker, there are no agents for direct imaging of 17B-HSD10 in living cells and tissues," the report states.

In the new study, researchers describe development of a compound with all the required properties for serving as such an agent. In laboratory tests on human cells, they showed that the new imaging agent lit up in the presence of 17B-HSD10 to permit non-invasive, real-time monitoring of the enzyme's activity. "This new imaging agent will be used to elucidate the biological functions of this important physiological marker," the study reported.

The journal article "Harnessing the Functional Plasticity of Enzymes: A Fluorogenic Probe for Imaging 17B-HSD10, an Enzyme Involved in Alzheimer's and Parkinson's Diseases" is scheduled for the Nov. 21 issue of the Journal of the American Chemical Society.

Adapted from materials provided by American Chemical Society.

[ZucchiniFlower]

Minireview

HSD17B10: A gene involved in cognitive function through metabolism of isoleucine and neuroactive steroids

.....This gene encodes HSD10, a mitochondrial multifunctional enzyme that plays a significant part in the metabolism of neuroactive steroids and the degradation of isoleucine.......

....HSD10 inactivates the positive modulators of GABAA receptors, and plays a role in the maintenance of GABAergic neuronal function......

Molecular Genetics and Metabolism
Volume 92, Issues 1-2, September-October 2007,

http://www.sciencedirect.com/science...e05248e6c2186f

http://beta.uniprot.org/uniprot/Q99714