Zeldagirl, ANA stands for "anti-nuclear antibody".

Some people make antibodies which react with components of the nuclei of their cells.

The "speckled" bit refers to which parts of the nuclei of your cells the antibodies are present. A speckled pattern can but need not be diagnostic of an auto-immune disease.

The "1:40" number that you mentioned is very low - it probably means that your anti-nuclear antibody levels are small so you don't have much to worry about.

For a more technical discussion of this, you might find the info here helpful; https://labtestsonline.org/understan.../ana/tab/test/ .