1: Neuroreport. 2007 Oct 29;18(16):1725-8.

Dopamine induces TNFalpha and TNF-R1 expression in SH-SY5Y human neuroblastoma
cells.

Gómez-Santos C, Francisco R, Giménez-Xavier P, Ambrosio S.

Biochemistry Unit, Department of Physiological Sciences II, University of
Barcelona - IDIBELL, Barcelona, Spain.

Cytotoxic concentrations of dopamine (100-500 microM DA) induce expression of
tumour necrosis factor receptor-1 (TNF-R1) and tumour necrosis factor-alpha
(TNFalpha) in SH-SY5Y human neuroblastoma cells. TNFalpha expression is
dose-dependent and can also be detected after 6-hydroxydopamine (6-OHDA) or
1-methyl-4-phenylpyridinium iodide (MPP) treatment. The expression of TNF-R1 is
also dose-dependent, but was not observed in 6-OHDA or MPP-treatment. Cells not
expressing TNF-R1 were insensitive to TNFalpha, whereas those treated with DA
showed a further decrease in viability when subsequently treated with TNFalpha.
Thus, DA treatment confers sensitivity to TNFalpha. The decrease of cell
viability caused by DA was in part prevented by neutralizing TNFalpha with
anti-TNFalpha. As TNF-R1 is increased in substantia nigra of Parkinsonian brains,
we suggest that nonvesiculated DA might also play a role in inducing TNF-R1
expression and predispose the neuron to the action of cytokines released in a
microglia-mediated inflammatory response.


PMID: 17921876 [PubMed - indexed for MEDLINE]