Green Tea's EGCGs reduces levels of MMP-9s.
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Biochim Biophys Acta. 2000 Mar 16;1478(1):51-60.
Matrix metalloproteinase inhibition by green tea catechins.
Demeule M, Brossard M, Page M, Gingras D, Beliveau R.
Laboratoire de Medecine Moleculaire, Hopital Sainte-Justine - UQAM, C.P. 8888,
Succursale centre-ville, Montreal, QC, Canada.
We have investigated the effects of different biologically active components
from natural products, including green tea polyphenols (GTP), resveratrol,
genistein and organosulfur compounds from garlic, on matrix metalloproteinase
(MMP)-2, MMP-9 and MMP-12 activities. GTP caused the strongest inhibition of the
three enzymes, as measured by fluorescence assays using gelatin or elastin as
substrates. The inhibition of MMP-2 and MMP-9 caused by GTP was confirmed by
gelatin zymography and was observed for MMPs associated with both various rat
tissues and human brain tumors (glioblastoma and pituitary tumors). The
activities of MMPs were also measured in the presence of various catechins
isolated from green tea including (-)-epigallocatechin gallate (EGCG),
(-)-epicatechin gallate(ECG), (-)-epigallocatechin (EGC), (-)-epicatechin (EC)
and (+)-catechin (C). The most potent inhibitors of these activities, as
measured by fluorescence and by gelatin or casein zymography, were EGCG and ECG.
GTP and the different catechins had no effect on pancreatic elastase, suggesting
that the effects of these molecules on MMP activities are specific.
Furthermore,
in vitro activation of proMMP-2 secreted from the glioblastomas cell line U-87
by the lectin concanavalin A was completely inhibited by GTP and specifically by
EGCG. These results indicate that catechins from green tea inhibit MMP
activities and proMMP-2 activation.
PMID: 10719174 [PubMed - indexed for MEDLINE]
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