Thanks for posting this information and thanks ROn for Activin A info.
This is so interesting and fits very well into what is already known.
Inflammation and immune dysregulation seem to be major promoting factors for PD. Microglia and T cells are the culprits for inflammation in PD. Several papers show that the presence of TGFbeta promotes T regulatory cells, these cells in turn dampen the microglial activation and this could potentially slow down PD. T reg cells seem to control everything, autoimmunity, cancer, inflammation and what not! Its time for cell therapy......
Cannot help it but say.......I wonder how much its going to cost for such therapies???
Girija
Quote:
Originally Posted by Ronhutton
I did a quick search and came across the following info.
It is only available for research
Ron
Recombinant Human Activin-A
Description :
Activin-A is a TGF-beta family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin-A in human colorectal tumors and in post-menopausal woman have been implicated in colorectal and breast cancers, respectively. The biological activities of Activin-A can be neutralized by inhibins and by the diffusible TGF-beta antagonist, Follistatin. Human Activin-A is a 26 kDa disulfide-linked homodimer of two beta A chains, each containing 116 amino acid residues.
Catalog #:
003-ACTIVINa
Source :
High-5 (BTI-Tn-5B1-4) Insect Cells
Formulation :
The sterile filtered solution was lyophilized from 50 mM Citric Acid, pH 3.0.
Stability :
The lyophilized protein is stable for a few weeks at room temperature, but best stored at -800C.
Reconstituted Human Activin-A should be stored in working aliquots at -800C.
Purity :
Greater than 95% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Reconstitution :
We recommend a quick spin followed by reconstitution in water to a concentration of 50 µg/ml (e.g., 5 µg/100µl) will yield a solution of 10 mM Citric Acid, pH 3.0. This solution can then be stored at room temperature or at 40C for 1 month. It is recommended that further dilutions be made in PBS containing 2 mg/ml albumin and stored at -800C.
Biological Activity :
The ED50 was determined by ability to inhibit the murine MPC-11 cells. The expected ED50 is 0.5 - 2.0 ng/ml.
PubMed Link :
Activin-A Human
Usage:
For research Purposes Only - Made using a cell BTI-Tn-5B1-4 under license from the Boyce Thompson Institute for Plant Research, Inc. Not for use in diagnostic or therapeutic procedures.
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