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07-09-2012, 02:37 PM | #1 | |||
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In Remembrance
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1. J Biol Chem. 2011 May 6;286(18):16504-15. Epub 2011 Mar 18.
Alternative mitochondrial electron transfer as a novel strategy for neuroprotection. Wen Y, Li W, Poteet EC, Xie L, Tan C, Yan LJ, Ju X, Liu R, Qian H, Marvin MA, Goldberg MS, She H, Mao Z, Simpkins JW, Yang SH. Department of Pharmacology and Neuroscience, Institute for Alzheimer's Disease and Aging Research, University of North Texas Health Science Center, Fort Worth, Texas 76107-2699, USA. Neuroprotective strategies, including free radical scavengers, ion channel modulators, and anti-inflammatory agents, have been extensively explored in the last 2 decades for the treatment of neurological diseases. Unfortunately, none of the neuroprotectants has been proved effective in clinical trails. In the current study, we demonstrated that methylene blue (MB) functions as an alternative electron carrier, which accepts electrons from NADH and transfers them to cytochrome c and bypasses complex I/III blockage. A de novo synthesized MB derivative, with the redox center disabled by N-acetylation, had no effect on mitochondrial complex activities. MB increases cellular oxygen consumption rates and reduces anaerobic glycolysis in cultured neuronal cells. MB is protective against various insults in vitro at low nanomolar concentrations. Our data indicate that MB has a unique mechanism and is fundamentally different from traditional antioxidants. We examined the effects of MB in two animal models of neurological diseases. MB dramatically attenuates behavioral, neurochemical, and neuropathological impairment in a Parkinson disease model. Rotenone caused severe dopamine depletion in the striatum, which was almost completely rescued by MB. MB rescued the effects of rotenone on mitochondrial complex I-III inhibition and free radical overproduction. Rotenone induced a severe loss of nigral dopaminergic neurons, which was dramatically attenuated by MB. In addition, MB significantly reduced cerebral ischemia reperfusion damage in a transient focal cerebral ischemia model. The present study indicates that rerouting mitochondrial electron transfer by MB or similar molecules provides a novel strategy for neuroprotection against both chronic and acute neurological diseases involving mitochondrial dysfunction. PMCID: PMC3091255 PMID: 21454572 [PubMed - indexed for MEDLINE]
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Born in 1953, 1st symptoms and misdiagnosed as essential tremor in 1992. Dx with PD in 2000. Currently (2011) taking 200/50 Sinemet CR 8 times a day + 10/100 Sinemet 3 times a day. Functional 90% of waking day but fragile. Failure at exercise but still trying. Constantly experimenting. Beta blocker and ACE inhibitor at present. Currently (01/2013) taking ldopa/carbadopa 200/50 CR six times a day + 10/100 form 3 times daily. Functional 90% of day. Update 04/2013: L/C 200/50 8x; Beta Blocker; ACE Inhib; Ginger; Turmeric; Creatine; Magnesium; Potassium. Doing well. |
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07-09-2012, 05:43 PM | #2 | ||
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It is antiinflammatory. It is a powerful antioxidant that causs no oxidative stress upon degrading, because of its affinity wirh Oxygen . It readily gives up an eectron to Oxygen, because it is an electron donor . The byproduct of this action produces Water for cellular hydration and active transport across the cell membrane. AND I have tried it.
I have contacted the company thatmakesit andreported my results to this point. I have also requested that they assist in funding a study . After having had this disease for twenty-three years It is practically unheard of to have 53 consecutive symptom free hours, but I DID. Last edited by michael7733; 07-09-2012 at 08:03 PM. Reason: correct the assembly of the last line |
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07-09-2012, 06:41 PM | #3 | ||
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Senior Member
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07-09-2012, 09:09 PM | #4 | ||
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Senior Member
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If anyone has a "recipe", please share. I dont' think there is a toxicity issue at the incredibly low dose one would be thinking about taking, but correct me if I'm mistaken. |
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07-10-2012, 05:07 PM | #5 | ||
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Junior Member
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Ted from Thailand has info on his page. That's the next thing I'm going to try. Amantadine isn't working very well
http://www.earthclinic.com/CURES/par...questions.html Shetawk |
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07-11-2012, 09:05 AM | #6 | ||
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Senior Member
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I don't know of ANYONE who has tried this....lots of people saying they were going to and asking how to do it, but no reports that I can find of anyone who used it and with what result. Anyone know anyone whose tried this? |
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07-11-2012, 04:56 PM | #7 | |||
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In Remembrance
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It is cheap, seemingly safe, readily available, and serious researchers say it works. Yet no one has tried it. I am going to later this year, but I think it is interesting to consider the question.
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Born in 1953, 1st symptoms and misdiagnosed as essential tremor in 1992. Dx with PD in 2000. Currently (2011) taking 200/50 Sinemet CR 8 times a day + 10/100 Sinemet 3 times a day. Functional 90% of waking day but fragile. Failure at exercise but still trying. Constantly experimenting. Beta blocker and ACE inhibitor at present. Currently (01/2013) taking ldopa/carbadopa 200/50 CR six times a day + 10/100 form 3 times daily. Functional 90% of day. Update 04/2013: L/C 200/50 8x; Beta Blocker; ACE Inhib; Ginger; Turmeric; Creatine; Magnesium; Potassium. Doing well. |
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