[reverett123]

1. J Biol Chem. 2011 May 6;286(18):16504-15. Epub 2011 Mar 18.

Alternative mitochondrial electron transfer as a novel strategy for
neuroprotection.

Wen Y, Li W, Poteet EC, Xie L, Tan C, Yan LJ, Ju X, Liu R, Qian H, Marvin MA,
Goldberg MS, She H, Mao Z, Simpkins JW, Yang SH.

Department of Pharmacology and Neuroscience, Institute for Alzheimer's Disease
and Aging Research, University of North Texas Health Science Center, Fort Worth,
Texas 76107-2699, USA.

Neuroprotective strategies, including free radical scavengers, ion channel
modulators, and anti-inflammatory agents, have been extensively explored in the
last 2 decades for the treatment of neurological diseases. Unfortunately, none of
the neuroprotectants has been proved effective in clinical trails. In the current
study, we demonstrated that methylene blue (MB) functions as an alternative
electron carrier, which accepts electrons from NADH and transfers them to
cytochrome c and bypasses complex I/III blockage. A de novo synthesized MB
derivative, with the redox center disabled by N-acetylation, had no effect on
mitochondrial complex activities. MB increases cellular oxygen consumption rates
and reduces anaerobic glycolysis in cultured neuronal cells. MB is protective
against various insults in vitro at low nanomolar concentrations. Our data
indicate that MB has a unique mechanism and is fundamentally different from
traditional antioxidants. We examined the effects of MB in two animal models of
neurological diseases. MB dramatically attenuates behavioral, neurochemical, and
neuropathological impairment in a Parkinson disease model. Rotenone caused severe
dopamine depletion in the striatum, which was almost completely rescued by MB. MB
rescued the effects of rotenone on mitochondrial complex I-III inhibition and
free radical overproduction. Rotenone induced a severe loss of nigral
dopaminergic neurons, which was dramatically attenuated by MB. In addition, MB
significantly reduced cerebral ischemia reperfusion damage in a transient focal
cerebral ischemia model. The present study indicates that rerouting mitochondrial
electron transfer by MB or similar molecules provides a novel strategy for
neuroprotection against both chronic and acute neurological diseases involving
mitochondrial dysfunction.

PMCID: PMC3091255
PMID: 21454572 [PubMed - indexed for MEDLINE]

[michael7733]

It is antiinflammatory. It is a powerful antioxidant that causs no oxidative stress upon degrading, because of its affinity wirh Oxygen . It readily gives up an eectron to Oxygen, because it is an electron donor . The byproduct of this action produces Water for cellular hydration and active transport across the cell membrane. AND I have tried it.

I have contacted the company thatmakesit andreported my results to this point. I have also requested that they assist in funding a study . After having had this disease for twenty-three years It is practically unheard of to have 53 consecutive symptom free hours, but I DID.

[lurkingforacure]

Originally Posted by michael7733

It is antiinflammatory. It is a powerful antioxidant that causs no oxidative stress upon degrading, because of its affinity wirh Oxygen . It readily gives up an eectron to Oxygen, because it is an electron donor . The byproduct of this action produces Water for cellular hydration and active transport across the cell membrane. AND I have tried it.

I have contacted the company thatmakesit andreported my results to this point. I have also requested that they assist in funding a study . After having had this disease for twenty-three years It is practically uhnhe

michaelard of ti have 53 consecutive symptom free hours but I DID.

That is so awesome, truly very happy for you. Can you tell us when you went off, how it happened, and what you are currently doing? Will this stuff extend your sinemet dose by 53 hours every time, or more as you go along? Or less? Please share with us how things are working for you, this is rather exciting for all of us!

[lurkingforacure]

Originally Posted by reverett123

1. J Biol Chem. 2011 May 6;286(18):16504-15. Epub 2011 Mar 18.

Alternative mitochondrial electron transfer as a novel strategy for
neuroprotection.

Wen Y, Li W, Poteet EC, Xie L, Tan C, Yan LJ, Ju X, Liu R, Qian H, Marvin MA,
Goldberg MS, She H, Mao Z, Simpkins JW, Yang SH.

Department of Pharmacology and Neuroscience, Institute for Alzheimer's Disease
and Aging Research, University of North Texas Health Science Center, Fort Worth,
Texas 76107-2699, USA.

Neuroprotective strategies, including free radical scavengers, ion channel
modulators, and anti-inflammatory agents, have been extensively explored in the
last 2 decades for the treatment of neurological diseases. Unfortunately, none of
the neuroprotectants has been proved effective in clinical trails. In the current
study, we demonstrated that methylene blue (MB) functions as an alternative
electron carrier, which accepts electrons from NADH and transfers them to
cytochrome c and bypasses complex I/III blockage. A de novo synthesized MB
derivative, with the redox center disabled by N-acetylation, had no effect on
mitochondrial complex activities. MB increases cellular oxygen consumption rates
and reduces anaerobic glycolysis in cultured neuronal cells. MB is protective
against various insults in vitro at low nanomolar concentrations. Our data
indicate that MB has a unique mechanism and is fundamentally different from
traditional antioxidants. We examined the effects of MB in two animal models of
neurological diseases. MB dramatically attenuates behavioral, neurochemical, and
neuropathological impairment in a Parkinson disease model. Rotenone caused severe
dopamine depletion in the striatum, which was almost completely rescued by MB. MB
rescued the effects of rotenone on mitochondrial complex I-III inhibition and
free radical overproduction. Rotenone induced a severe loss of nigral
dopaminergic neurons, which was dramatically attenuated by MB. In addition, MB
significantly reduced cerebral ischemia reperfusion damage in a transient focal
cerebral ischemia model. The present study indicates that rerouting mitochondrial
electron transfer by MB or similar molecules provides a novel strategy for
neuroprotection against both chronic and acute neurological diseases involving
mitochondrial dysfunction.

PMCID: PMC3091255
PMID: 21454572 [PubMed - indexed for MEDLINE]

I learned about it here, and we talked to our neuro about it long, long ago. Aside from commenting that they used to play tricks on first year medical students with it (sneak it into their drink, and then laugh when they went to the bathroom....ha ha ha), he said you would have to dilute it so much it would be near impossible to calculate. I seem to remember something along the lines of one dropperful per swimming pool (Olympic size, no less)...kinda hard to do.

If anyone has a "recipe", please share. I dont' think there is a toxicity issue at the incredibly low dose one would be thinking about taking, but correct me if I'm mistaken.

[shetawk]

Ted from Thailand has info on his page. That's the next thing I'm going to try. Amantadine isn't working very well

http://www.earthclinic.com/CURES/par...questions.html

Shetawk

[lurkingforacure]

Originally Posted by shetawk

Ted from Thailand has info on his page. That's the next thing I'm going to try. Amantadine isn't working very well

http://www.earthclinic.com/CURES/par...questions.html

Shetawk

I went to the link and there were quite a few PWP discussing this on that website, different concentrations and how to make it....yet no posts about whether it was working for anyone. It makes me wonder why.

I don't know of ANYONE who has tried this....lots of people saying they were going to and asking how to do it, but no reports that I can find of anyone who used it and with what result. Anyone know anyone whose tried this?

[reverett123]

It is cheap, seemingly safe, readily available, and serious researchers say it works. Yet no one has tried it. I am going to later this year, but I think it is interesting to consider the question.

Originally Posted by lurkingforacure

I went to the link and there were quite a few PWP discussing this on that website, different concentrations and how to make it....yet no posts about whether it was working for anyone. It makes me wonder why.

I don't know of ANYONE who has tried this....lots of people saying they were going to and asking how to do it, but no reports that I can find of anyone who used it and with what result. Anyone know anyone whose tried this?