http://www.ncbi.nlm.nih.gov/pubmed/24217017

From the referenced paper:

"OBSERVATIONS Two patients received intrastriatal grafts of human fetal ventral mesencephalic tissue, rich in dopaminergic neuroblasts, as restorative treatment for their Parkinson disease. To evaluate the very long-term efficacy of the grafts, clinical assessments were performed 18 and 15 years posttransplantation. Motor improvements gained gradually over the first postoperative years were sustained up to 18 years posttransplantation, while both patients have discontinued, and remained free of any, pharmacological dopaminergic therapy. CONCLUSIONS AND RELEVANCE The results from these 2 cases indicate that dopaminergic cell transplantation can offer very long-term symptomatic relief in patients with Parkinson disease and provide proof-of-concept support for future clinical trials using fetal or stem cell therapies."

Impressive!

Assuming the results can be replicated, a key issue will be the complexity and cost of the surgery.

Does any one know how the cost of this procedure will compare with the cost of DBS?

What is slowing down the use of stem cell grafts?

John

Are they saying that these 2 patients are off their meds and have been for 15 and 18 years?!! This is incredible! Where is the gas pedal for this? Oh wait a minute. NIH is involved so we wont have any big pharma to declare that this was somehow not reliable. Or another Amgen debacle! Imagine what it would do if this therapy went mainstream!

there were also some very bad results. i think each operation used material from many fetuses so that obviously was very controversial.
canadians and europeans are hopefully starting new trials soon.


http://www.biosciencetechnology.com/...g#.Ut7YnLSIaXI
http://clinicaltrials.gov/show/NCT01898390

Below is an exerpt from the posted study. I am not a scientist, but it seems to me, 2 of these patients have been essentially cured of Parkinson's. This is a 15 to 18 year post assessment but showing improvements after 4 years. Where has the NIH been on this!? Stem cell science must have come a long way over 18 years. Why aren't they picking this trial apart to find out what worked and what didn't? My wife has had PD now for 11-12 years and her quality of life is awful. Ten years ago, I believed there would be a treatment much better than l-Dopa by now.
Something is wrong.
John

We describe 2 patients (patients 7 and 15 in the Lund series) who received intrastriatal transplantations of human fetal ventral mesencephalic tissue, rich in dopaminergic neuroblasts, as an experimental treatment for their PD. Throughout their disease course, both patients experienced excellent responses to levodopa treatment. However, both subsequently developed disabling “on-off” fluctuations with accompanying severe levodopa-induced dyskinesias (LIDs). The patients were clinically followed up at the National Hospital for Neurology and Neurosurgery, Queen Square, London, England. Transplantation was performed in Lund, Sweden. The grafts were placed bilaterally using the Rehncrona and Legradi transplantation instrument in a staged manner. A magnetic resonance imaging–guided stereotactic technique was used for targeting the putamen (patient 7) and the putamen and caudate nucleus (patient 15), at the ages of 49 and 54 years and after disease durations of 10 and 12 years, respectively.
Results



Following transplantation, patient 7 experienced significant motor benefits, which gradually emerged over the course of 4 years. He was able to stop levodopa treatment 26 months after the first transplantation, by which time the “on-off” phenomena had virtually disappeared and his practically defined “off” motor UPDRS score had decreased by 38% compared with baseline score. By the fifth postoperative year, all dopaminergic agents had been withdrawn while the patient’s motor status continued to improve. At his last assessment, 18 years postgrafting, the patient demonstrated sustained motor benefits, scoring 22 on the UPDRS motor examination, reported no fluctuations, and remained free of any pharmacological dopamine replacement therapy. The patient continues to be independent in all activities of daily living . While his speech remains dysarthric and hypophonic, his swallowing is normal, and falls or freezing have not emerged as a problem.
Postoperatively, this patient reported dyskinesias during “off” medication phases (graft-induced dyskinesias [GIDs]).z Levodopa-induced dyskinesias were still present following levodopa administration, prompting reduction in levodopa replacement therapy, which successfully led to their improvement. In the early postoperative years, GIDs persisted despite discontinuation of all dopaminergic agents, were mild to moderate in severity, and caused no distress or disability. Nonpainful dystonic posturing of his right foot also developed after transplantation. At the most recent follow-up, he had GIDs of moderate degree that he felt were to some extent helped by amantadine hydrochloride and buspirone hydrochloride. His GIDs were still less severe in comparison with his LIDs pretransplantation. His left foot dystonia partially interfered with his walking, which, nonetheless remained independent and safe, with good arm swing bilaterally.


During the first 2 years after transplantation, patient 15 showed no improvement in UPDRS motor scores in the “practically defined off” state. However, “on” periods were prolonged and the patient’s self-reported frequency and severity of motor fluctuations diminished, allowing a 66% reduction in his daily levodopa equivalent dose. Improvements in motor function became more evident from the fourth year postgrafting, by the end of which he was able to stop all dopaminergic medication, given that “on” and “off” conditions were almost indistinguishable. Assessment 15 years posttransplantation demonstrated preserved motor benefits. During the motor examination, the patient had minor rigidity and bradykinesia, normal gait, and intact postural reflexes. He remained free of dopaminergic medication. He was aware of some diurnal variation of his motor function but remained independent in all activities of daily living . Freezing and falls were frequent before grafting, even during the “on” condition, whereas the patient now reports very rare freezing episodes and no falls.
During the first postoperative year, his LIDs markedly decreased in severity and duration, but the patient noticed spontaneous dyskinesias independent of levodopa intake. These have persisted over the ensuing years but have had no functional impact. At the latest follow-up assessment, the patient’s GIDs mainly involved his legs and were of mild to moderate severity. The patient found that these were only modestly helped by amantadine. Graft-induced dyskinesias (despite being his major current concern) remained milder than the severe biphasic and peak-of-dose LIDs he experienced pregrafting, which sometimes forced him to lie on the floor.


The Non-Motor Symptoms Questionnaire revealed a number of nonmotor symptoms for both patients that did not require pharmacological treatment . While nonmotor questionnaires were not used pretransplantation, so that direct comparison is not possible, most of the current nonmotor concerns had also been present prior to grafting. At latest neuropsychological assessment, patient 7 had evidence of some decline in executive function (semantic verbal fluency and Trail Making Test) and episodic memory and learning of words. Patient 15 had evidence of some decline in executive function (Wisconsin Card Sorting Test, phonemic verbal fluency, and Stroop Test), episodic memory and learning of words, and attention. Nevertheless, there was no indication of any clinically significant cognitive decline. At the time of the latest follow-up, the Mini-Mental State Examination score was 30 of 30 in both patients.


No coding mutations were identified in any of the extensive genetic tests performed for either patient. In line with the clinical postoperative improvements, an increase in striatal 18F-Dopa uptake was observed after transplantation .


DISCUSSION




This is an exerpt


This study presents the very long-term clinical outcomes in 2 patients with PD treated with intrastriatal fetal ventral mesencephalic grafts. Clinical assessments 18 and 15 years posttransplantation demonstrate sustained benefits, with motor scores still lower than their preoperative baselines. Despite each patient having nearly 30 years of PD with troublesome fluctuations and dyskinesias prior to transplantation, both patients now present with very mild symptoms and have been independent of any pharmacological dopaminergic treatment for more than 10 years. Neither patient had axial impairment nor dementia despite the presence, in patient 15, of freezing and falls prior to transplantation, features that often herald a progressive decline in independent function. These data are of course uncontrolled; therefore, the very long-term clinical natural history of their disease in the absence of surgery cannot be reliably inferred. However, the sustained motor benefits agree well with the gradual, complete normalization of 18F-dopa uptake (as a measure of dopaminergic innervation) and carbon 11–labeled raclopride binding (as a measure of drug-induced dopamine release) in the grafted putamen of both patients. Moreover, the course of PD is generally relentlessly progressive, and the limited progression of disability in these 2 individuals since grafting is not usually achieved by other conventional therapeutic strategies. It is well recognized that, in some subjects with young-onset disease and recessively inherited parkinsonism due to Parkin mutations, disease may progress relatively slowly. However, our patients actually improved, as opposed to progressing slowly, and full testing for such mutations was negative in both of these patients. Possible factors that may relate to the prolonged remarkable beneficial effects of transplantation seen in these patients are their young age at onset, preserved preoperative ventral striatal dopaminergic uptake, and preserved levodopa response.


There are, however, a number of points to be made in relation to these observations. These patients represent 2 of a total of 18 patients (3 were cases with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine–induced parkinsonism) who received fetal cell transplantation in Lund, Sweden, on an open-label basis from the late 1980s to the mid-1990s. Short- to mid-term outcomes have been previously reported in detail. An overview analysis of the results of the whole cohort has been presented by Lindvall and Björklund and in a meta-analysis article by Barker et al, showing variable but overall favorable outcomes posttransplantation. In addition to the 2 individuals described in the current study, at least another 2 of this cohort (patient 4 and patient 13) were reported to have an equally favorable outcome 10 or more years posttransplantation. The remainder of the cohort have either died or been lost to long-term follow-up.


Graft-induced dyskinesias occurred in both patients but did not have a significant functional impact and their occurrence was outweighed by the beneficial effects on motor function gained over the years. Nevertheless, GIDs represent a serious adverse event that can be debilitating but seem to be helped by deep brain stimulation of the internal segment of the globus pallidus. Neither patient has been sufficiently troubled by their GIDs to consider pallidal deep brain stimulation as a treatment. Undoubtedly, understanding and finding ways to avoid GIDs remains of major importance for the future of dopaminergic cell therapies.


Postmortem studies of brains from patients with PD receiving fetal neural grafts over a decade prior to death have shown that some of the grafted dopamine neurons exhibit Lewy bodies. Although the proportion of cells displaying Lewy bodies is only reported to be in the range of 2% to 8%, as many as 80% exhibit increased levels of soluble α-synuclein in the cell bodies, a change consistent with premature aging of the cells. More detailed follow-up studies suggest that over time some of the grafted neurons progressively express reduced levels of the dopamine transporter and tyrosine hydroxylase, which further supports the notion that the disease process directly impacts the grafted cells. It is likely that the 2 cases described in this report also have similar changes in their grafted neurons. In that context, it is particularly encouraging that the grafts still continue to exert positive functional effects.


The gradual emergence of clinical improvements in both patients, albeit anticipated given the nature of the intervention, is highly relevant with respect to future cell therapy trial designs, particularly in relation to the variable long-term natural history of PD. It may be that the major effects of dopaminergic cell replacement with respect to motor and nonmotor symptom control only become fully apparent with long-term follow-up. Whether future transplantation protocols should also target extrastriatal nondopaminergic systems to achieve more widespread benefits in motor and nonmotor features of PD not dopaminergic in origin remains a subject under consideration. In this report, we are aware that only 2 individuals are included and that these represent 2 particularly successful cases, and thus, any conclusions should be drawn with caution. Nevertheless, we would suggest that these patients, together with a few other cases exhibiting similarly long-term favorable outcomes, provide support that dopaminergic cell transplantation may offer a substantial and very long-lasting compensatory effect in PD. Our results provide encouragement for basic science and clinical studies that strive to develop a clinically competitive stem cell–based dopaminergic cell therapy for PD.