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04-21-2014, 12:29 PM | #1 | ||
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http://www.cell.com/cell-stem-cell/a...2814%2900137-4
Highlights •Adult cell reprogramming via SCNT is not successful via the conventional protocol •Improved success was achieved with a recently developed approach •hESCs were derived via SCNT from 35- and 75-year-old males Summary Derivation of patient-specific human pluripotent stem cells via somatic cell nuclear transfer (SCNT) has the potential for applications in a range of therapeutic contexts. However, successful SCNT with human cells has proved challenging to achieve, and thus far has only been reported with fetal or infant somatic cells. In this study, we describe the application of a recently developed methodology for the generation of human ESCs via SCNT using dermal fibroblasts from 35- and 75-year-old males. Our study therefore demonstrates the applicability of SCNT for adult human cells and supports further investigation of SCNT as a strategy for regenerative medicine. |
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"Thanks for this!" says: | lab rat (04-21-2014) |
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