Quote:
Originally Posted by kiwi33
John, you have asked some hard questions which I will try to answer as best that I can.
The issue is: can it be measured?
Yes, alpha-synuclein levels can be measured in biological fluids (CSF, plasma, etc) - I would do this using an immunoassay. An immunoassay could also be used to measure intra-cellular alpha-synuclein levels in whatever kind of cells after those cells have been lysed, which means releasing their contents.
And, better still, is there a DIY way of measuring it and its misfolding?
The short answer to these questions is "No". Immunoassays and ways of measuring alpha-synuclein misfolding both need access to expensive instruments which are not generally available DIY.
For instance, aggregations of alpha-synuclein, Lewy bodies, can be seen in a microscope.
Lewy bodies can be seen in a microscope. However, that does not show that they contain alpha-synuclein - it needs immunocytochemistry, which I mentioned in one of my contributions above in this discussion.
I hope that you find my answers to your questions helpful.
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I found an image of alpha synuclein but sure would like to know what the magnification factor is. I sure would like to take a look at my alpha synuclein. I wonder if there was a way to do that!
https://www.google.com/search?q=amyl...%3B1500%3B1500
Here's another interesting image
https://www.google.com/search?q=alph...l%3B1000%3B750