Parkinson's Disease Tulip


advertisement
Reply
 
Thread Tools Display Modes
Old 06-22-2015, 02:48 PM #1
girija girija is offline
Member
 
Join Date: Nov 2006
Location: southern tip of west coast
Posts: 582
15 yr Member
girija girija is offline
Member
 
Join Date: Nov 2006
Location: southern tip of west coast
Posts: 582
15 yr Member
Default PD-alpha synluein-mitochondria: an exciting animal study

link added
http://www.upmc.com/media/NewsReleas...e-therapy.aspx

Last edited by Chemar; 06-22-2015 at 05:46 PM. Reason: copyright rticle copy pasted...I added the link instead
girija is offline   Reply With QuoteReply With Quote
"Thanks for this!" says:
badboy99 (06-22-2015), BreezyRacer (06-22-2015), GerryW (06-22-2015), kiwi33 (06-22-2015), lab rat (06-22-2015), Lexiegirl (06-22-2015), olsen (06-29-2015), shcg (06-24-2015)

advertisement
Old 06-22-2015, 04:26 PM #2
wxxu wxxu is offline
Member
 
Join Date: Dec 2011
Location: East Pennsylvania
Posts: 105
10 yr Member
wxxu wxxu is offline
Member
 
Join Date: Dec 2011
Location: East Pennsylvania
Posts: 105
10 yr Member
Default

Quote:
Originally Posted by girija View Post
News Release Search
GO
UPMC/University of Pittsburgh Schools of the Health Sciences


Gene Therapy Prevents Parkinson’s Disease in Animal Model, Says Pitt Study
http://www.upmc.com/media/NewsReleas...e-therapy.aspx
.
This is meaningful. Hope they can move to clinic quickly to prove it. Thank you for this!

Last edited by Chemar; 06-22-2015 at 05:48 PM. Reason: quoteed post had to be edited for copyright reasons
wxxu is offline   Reply With QuoteReply With Quote
Old 06-22-2015, 07:18 PM #3
kiwi33's Avatar
kiwi33 kiwi33 is offline
Grand Magnate
 
Join Date: Jan 2015
Location: Sydney, Australia.
Posts: 3,093
8 yr Member
kiwi33 kiwi33 is offline
Grand Magnate
kiwi33's Avatar
 
Join Date: Jan 2015
Location: Sydney, Australia.
Posts: 3,093
8 yr Member
Default

This looks very encouraging to me.

The paper referred to in the link is available here; http://www.jci.org/articles/view/64502 .
__________________
Knowledge is power.
kiwi33 is offline   Reply With QuoteReply With Quote
Old 06-22-2015, 11:58 PM #4
Lana Lana is offline
Junior Member
 
Join Date: Feb 2014
Location: San Francisco Bay Area
Posts: 28
10 yr Member
Lana Lana is offline
Junior Member
 
Join Date: Feb 2014
Location: San Francisco Bay Area
Posts: 28
10 yr Member
Default a pinch of salt ? - opposite results in a monkey experiment

Quote:
Originally Posted by girija View Post
Below is a poster presented at 2015 MDS meeting at San Diego:

rAAV-Mediated Reduction of Alpha-Synuclein in the Substantia Nigra of the African Green Monkey Results in Neurodegeneration


Fredric P. Manfredsson, D. Eugene Redmond Jr., Jack W. Lipton, Ryan M. Malpass, Timothy J. Collier. Translational Science and Molecular Medicine, Michigan State University College of Human Medicine, Grand Rapids, MI; Department of Psychiatry & Neurosurgery, Yale School of Medicine, New Haven, CT

Parkinson Disease (PD) is characterized by the loss of midbrain nigrostriatal dopaminergic neurons and the presence of proteinacious inclusions known as Lewy bodies. Lewy bodies are highly enriched in the protein alpha-synuclein (α-syn). Mutations or multiplication of the α-syn gene are also implicated in some familial forms of PD. Thus, α-syn has been proposed to directly contribute to dopamine (DA) neuron loss in sporadic and familial forms of PD. The predominant hypothesis posits that α-syn-mediated pathology arises due to a toxic gain-of-function. Hence, some therapeutic strategies are aimed at reducing α-syn protein from DA neurons. However, our own efforts to reduce α-syn load in midbrain DA neurons of rodents caused enhanced DA neurodegeneration. This suggested that the reduction of α-syn protein can also be toxic, and that a certain level of α-syn is crucial to DAergic neuron survival. As a result of this observation we propose that α-syn is not a toxic species in PD per se; but rather that α-syn aggregation decreases the pool of soluble α-syn within a neuron to sufficiently impair cellular function and produce pathology. In the current study we expanded our investigation to the non-human primate.

African Green monkeys (n=1) were injected unilaterally in the substantia nigra (SN) with low (5x1012 vector genomes (vg)/ml) or high (2x1013vg/ml) titer AAV2/5 expressing a shRNA designed against a-syn or scrambled shRNA as control (total of 4 subjects). Animals were monitored for behavioral deficits indicative of nigrostriatal denervation for three months until sacrifice. Following sacrifice, brains were analyzed for striatal catecholamine content and neuropathology of the SN.

The subject treated with high-dose a-syn shRNA exhibited a progressive deficit in a summary score of healthy behaviors, but no increase in overt parkinsonian signs. Analysis of tissue indicated that all a-syn shRNA treated animals exhibited reduced striatal DA levels as compared to the intact hemisphere. A stereological count of midbrain tyrosine hydroxylase positive (TH+) neurons indicated that neurodegeneration was preferentially present in the ventral tier of a-syn shRNA treated SNs, whereas the dorsal tier displayed modest neurodegeneration. The ventral tegmental area remained intact. Scrambled shRNA treated subjects showed no behavioral or neural pathology. Transduction, as measured by the marker GFP (transduction marker), was observed throughout the SN in control subjects. In contrast, GFP was only seen in dorsomedial neurons of a-syn shRNA treated animals, ostensibly due to the loss of DA neuron loss in the ventral tier. Additionally, a-syn shRNA treatment caused a loss of TH phenotype in some surviving neurons as indicated by the observation of several TH-/neuromelanin+ neurons.

Our results indicate that non-human primate nigral dopamine (particularly ventral tier) neurons require a-syn for survival, and that the experimentally-induced neuron loss is not a product of non-specific shRNA toxicity.
Lana is offline   Reply With QuoteReply With Quote
"Thanks for this!" says:
GerryW (06-23-2015), lab rat (06-23-2015), wxxu (06-23-2015)
Reply

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


Similar Threads
Thread Thread Starter Forum Replies Last Post
Alpha-Glycerolphosphocholine (Alpha-GPC) vs CDP Choline ? melon Peripheral Neuropathy 0 09-14-2011 10:30 PM
New study adds an intriguing bit to als/mitochondria puzzle BobbyB ALS News & Research 0 09-04-2008 01:48 PM


All times are GMT -5. The time now is 06:51 AM.

Powered by vBulletin • Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.

vBulletin Optimisation provided by vB Optimise v2.7.1 (Lite) - vBulletin Mods & Addons Copyright © 2024 DragonByte Technologies Ltd.
 

NeuroTalk Forums

Helping support those with neurological and related conditions.

 

The material on this site is for informational purposes only,
and is not a substitute for medical advice, diagnosis or treatment
provided by a qualified health care provider.


Always consult your doctor before trying anything you read here.