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-   -   Protein Electrophoresis questions (https://www.neurotalk.org/peripheral-neuropathy/184365-protein-electrophoresis-questions.html)

QJayde5 03-03-2013 11:16 AM

Quote:

Originally Posted by glenntaj (Post 962389)
It does still sound like you to need to have the more advanced immunofixation electrophoresis to enumerate and examine the components of your gamma globulins. From what you've reported that has not been done, as you would have figures for IgA, IgG and IgM concentrations as well as more specific analysis to see if any monoclonal proteins were detected.

My report doesn't mention IgA, IgG, or IgM. How would the report mention the immunofixation electrophoresis? Is there an example online I could see?

Also, do you have any information on Urine Protein Electrophoresis? I also had that test done.

glenntaj 03-04-2013 08:18 AM

The urine protein electrophoresis--
 
--would indicate if there were any Bence-Jones proteins (which are the urinary breakdown of monoclonals) and/or any light chain protein of the lambda or kappa class. If there are none of these, that is a good thing.

An immunofixation electrophoresis would be definitely demarcated in a lab report--it might be abbreviated IFE--and it would generally come with figures for amounts of IgA , IgM, and IgG along with reference ranges.

Here's one of my favorite listings of lab tests with reference ranges--look on page 61/62:

http://www.hosp.uky.edu/ClinLab/report.pdf

QJayde5 03-04-2013 11:14 AM

1. My Serum Protein Electrophoresis says it has "RFX to Immunofixation." But, I don't see anything about IgA , IgM, and IgG. Could it be they didn't find anything to comment on?

2. How would the Urine Protein Electrophoresis show Bence-Jones proteins? Different proteins showed up on mine. For example, Beta Globulin is 1.1 mg/dl. Albumin is 0.9 mg/dl. I don't see any ranges, except for the Total Protein.

glenntaj 03-05-2013 07:23 AM

Your serum protein electrophoresis--
 
--did not also result in an immunofixation and gamma globulin enumeration being also done, apparently. If these was done, results would have been included.

Same with the Bence Jones proteins--apparently in your urinalysis they did a protein breakdown by type of protein--basically, albumin and globulins--but not a FURTHER breakdown by type of gamma globulin. Again, if that had been done, there would have been a notation such as "no Bence Jones proteins detected" or "no light chains detected".

QJayde5 03-06-2013 01:44 PM

Thank you for answering me. I hope you don't mind me asking questions.

1. My blood test mentions immunofixation. But there is no mention of the IgG, IgA, etc. That seems strange. The results mentioned are: Total Protein; the types of protein in g/dl (Albumin, Alpha 1 Globulin, etc); A/G ratio; and the protein fractions with %. There is also an interpretation.

2. My urine tests mention: Total Protein; Protein 24 hr calculated; the types of protein in mg/dl (Albumin, Alpha 1 Globulin, etc); and the protein fractions with %. It seems the Beta Globulin is higher than the other proteins. The fraction is 30% and 1.1 mg/dl. Would that indicate anything?

I can't seem to get a hold of the doctor who ordered the tests. I go to a clinic. They are only there 2 times a week. I have an appointment, but it's not until June.

glenntaj 03-07-2013 07:24 AM

Yes, it does seem strange--
 
--unless you don't have the complete results.

By definition, an immunofixation electrophoresis is concerned with enumerating and typing the IMMUNE proteins--which are the gamma globulins, or antibodies, of the Ig class, so it should have information about amount and type of IgA, IgG, IgM at least.

Is there nobody at the clinic you can ask the question of besides the original physician you saw?

QJayde5 03-07-2013 02:30 PM

For the blood electrophoresis, I called LabCorp (where the test was sent). The person I spoke to said the immunofixation part was not done. She said it was because an M Spike was not observed. I guess that's why the IgA, IgG, IgM are not on my results.

I also asked the lab about the urine electrophoresis. She didn't give me any range for anything like the globulins and fractions. She said there isn't any. I found some info on the LabCorp site. But, It doesn't have any range either. Here is the site: http://tinyurl.com/bdrbz5z

There is no one else at the clinic I can ask. I left 2 messages. The nurse I spoke with was kind of rude. I could ask my primary care doctor or another one.


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