Thanks for posting this information, Janieg!

Did your convos with Dr Wolf involve information about the multivitamin transporter? If that is not working properly, biotin from the diet will not get to the tissues either. I have to wonder if genetic information even exists at this time?

No, it didn't cover that topic. Sorry.

I didn't want to take up too much of his time, but I did ask him whether biotin played any role in alcohol metabolism. I suspect my inability to properly metabolize alcohol might be a clue as to my problem. Maybe a deficiency or low levels of another enzyme in concert with lower levels of biotinidase is contributing to my issues. He said he didn't know of biotin involvement in that area, though.

*** Just putting this here for giggles...

Alcohol is eliminated from the body by various metabolic mechanisms. The primary enzymes involved are aldehyde dehydrogenase (ALDH), alcohol dehydrogenase (ADH), cytochrome P450 (CYP2E1), and
catalase. Variations in the genes for these enzymes have been found to influence alcohol consumption, alcohol-related tissue damage, and alcohol dependence.

Hello,
I just found this website by searching for c.1330G>C. This is my first comment, please forgive me if I am not doing it right. I too have that gene found through a genetic screen. I have numerous health nuiances, one of which is difficulty metabolising alcohol. Interestingly three other women in my family have the same thing. I'm very interested to know what other similarities I have with someone with the same gene. I understand the thread is old, I hope this is received. Also did you find a balance in managing symptoms?? If so how?

Hello Alexia,

Welcome to NeuroTalk. Despite the thread being old, I received notification of your response thanks to having email notifications being enabled on the thread.

I'm happy you posted. I feel rather alone with this gene mutation. I assume you have only one copy and not two? Having two copies is much more problematic.

Since making sure I supplement with biotin everyday, I have not been hypoglycemic once. It was definitely causing me metabolism problems. Unfortunately, I've seen no improvement with my ability to metabolize alcohol, nor has it improved my neuropathy problems.

The latter, however, I've just recently discovered are associated with histamine overload, which is a whole nightmare in itself.

Have you had your biotinidase enzyme levels tested?

Jane

Hi OP, at first glance all I can say is in my long life, I see no saving graces to consuming and putting alcohol in our bodies....... I can't speak about the gene issue but lived with too much alcohol in my family. Thank goodness both parents didn't imbibe.... Good luck.

Thank you for replying, this is great. Yes, I just have the one copy.

Histamine is something for me to look into, thank you. It may even be causing some of my other problems.

No enzyme levels yet, my next appointment is in a few weeks. There's only one metabolic clinic here and I think that test might also be harder to find where I am.

My symptoms being investigated are: neuropathy, alcohol sensitivity (seems to run in family), cognition, fatigue, ADD, anovulation, itchy skin bumps, dry skin, mouth bumps, twitching, general gut, headaches, sore throat, alopecia, weight/hunger, cholesterol, zinc, mood... not sure what else. Most of my symptoms come and go which makes it difficult to be assessed.

I've been taking biotin for 8 months and take between 300mcg and 10000mcg, I personally think the upper limit is too much for me. Some of my symptoms (especially waking in the morning) are better but not perfect. Are you at liberty to say what amount works for you personally?

Currently trying to start a family so the unpredictable ovulation is my main sore point if it's related.

Fun, fun, fun. Haha.

Sorry for the delay in responding. I didn't receive notifications of the new posts for some reason.

I'm taking 5 mg of Meribin biotin/day. It's a highly bioavailable form of biotin, and the brand recommended for profoundly deficient biotinidase people. It's very expensive, though, and probably overkill. I'm at the end of my last bottle now, and am going to switch to a less expensive form. I'll know pretty quickly if it doesn't do the job.

My functional medicine doctor ran my 23andme results through an analyzer that spit out a ton of "SNP" data. I'm highly mutated on my ALDH2 gene, which is a gene involved in alcohol metabolism. I'm almost certainly deficient in the enzyme it produces that is phase 2 of alcohol breakdown. Without enough of that enzyme, you'd be left with a substance called acetaldehyde cruising through your veins. It's effects are well-documented, and I'm sure this is the root of my problem with alcohol consumption.

Cardiovascular effects of acetaldehyde accumulation after ethanol ingestion: their modification by beta-adrenergic blockade and alcohol dehydrogenase inhibition - PubMed

Accumulation of acetaldehyde in blood was accompanied by marked increases in heart rate (53%) and cardiac output (78%) as well as by decreases in diastolic arterial blood pressure (19%) and peripheral vascular resistance (46%).

With regard to investigating histamine as a possible cause of problems, the best thing you can do is note your activity, especially with regard to what you've eaten, prior to a flare of symptoms.

Sounds like a thorough doctor, I am glad you found a good one to help. I have my next appointment at the start of November.

I was going to message to put an idea forth regarding the alcohol, though this new information makes alot of sense. I suppose in your circumstance could you try the alcohol dehydrogenase blocker or what was suggested in the article to improve your symptoms?
I must admit that most times I am skim reading information, it's hard to read and remember alot of the time so it's how I manage. Some times I wonder how I got through a science degree, haha. Hoping I can eventually have more good days than bad though!

I stumbled across an article that I interpreted to mean c.1330C>G gene can result in the incorrect conversion of a certain protein into histidine. As this is used in histamine synthesis I wondered if c.1330C>G would mean more histamine problems. Maybe far-fetched.

I hope that you can manage ALDH2 and improve your tolerance.

I found something suggesting biotin is important in utilising B12, it wasn't a journal article so I need to look into it more. I have normal B12 levels though this point interested me as doctors through the years keep testing me even when I say my levels are normal.

I think I will try the Meribin, it's even more expensive here as we don't stock in Australia. I have tried 5 different brands and different levels. I have an idea about what I think doesn't work but I wouldn't want to say as I mix dose/brand frequently. Currently trying Caruso's and Natrol.

I've done several food diaries, though admittedly it was always looking for food intolerance not histamine problems. Will keep it in mind for next Naturopath appointment.

Currently my grandfather, and either my mother or cousin are going to do DNA tests. We want to find out if the gene came from my mum or dad and if my grandmother (deceased) had it. Retrospectively grandmother having the gene would explain a lot. Hope that the familial data answers some questions in general as well. Will ofcourse share the information.

Kind regards
Alexia

Some SNPs in the multivitamin transporter (SLC5A6) have been reported but as far as I can see their possible clinical significance is not yet known.

See the "Genomic Variants" section in SLC5A6 Gene - GeneCards | SC5A6 Protein | SC5A6 Antibody for more on this.

I'm going to do some intense pouring over of my genetic data...for lack of any other options right now. Just to give you an idea of what's involved when you test with an outfit like Genos Research...

To stay out of trouble with the FDA, they only report on your variants that have some publicly available and "trusted" information on them. They tell you whether you have one or two copies of the variant and then categorize it as Pathogenic, Likely Pathogenic, VUS (Variant of Undetermined Significance), Likely Benign, or Benign based on the overall findings of the available reports. They then link to all the reports.

I have 3749 variants with this breakdown:

Pathogenic - 34
Likely Pathogenic - 6
VUS - 75
Likely Benign - > 500
Benign - > 500

They also give you a helpful histogram chart showing the categorization of the available reports and how many reports are available. For example, the biotinidase variant was right at the top of the list because it had the most reports with 15, and the histogram showed all 15 reports were red or pathogenic. Everyone was in violent agreement that this was a bad variant. With other ones, you'll see some disagreement between reports on whether they're bad or not.

You can also sort them by population prevalence which is interesting. For example, here's one that that is only present in 0.180% - 0.238% of the population with four reports available, three of them saying pathogenic, and one saying likely pathogenic.

NM_001003841.2(SLC6A19):c.517G>A (p.Asp173Asn) AND Neutral 1 amino acid transport defect

And if I click on the reports, I see a bunch of gobbledygook, but look for some words in plain English that might help. In this case, "Hartnup disease" was stated which I can Google.

Hartnup disease - Genetics Home Reference

"Hartnup disease is a condition caused by the body's inability to absorb certain protein building blocks (amino acids) from the diet. As a result, affected individuals are not able to use these amino acids to produce other substances, such as vitamins and proteins. Most people with Hartnup disease are able to get the vitamins and other substances they need with a well-balanced diet."

After further reading, I see that nicotinamide or niacin supplements are recommended for people with this disorder. Maybe I'll add one of those to my arsenal.

Such fun.

As an addendum, I should mention that since I started taking biotin, a bad patch of skin I've had for about a decade has cleared up. Woohoo!